Northern blotting analysis of human microRNA was conducted and showed that the XENOPURE Blood Small RNA Purification Kit isolated 160% more hsa-miR-486 and 340% more hsa-miR-92a than competitors’ kits.
Advantages
- High Yields with high purity
- Efficient enrichment of small RNA
- Excellent at removing gDNA
- No protease treatment required
- Simultaneous extraction of mRNA and miRNA
The XENOPURE Blood Small RNA Purification Kit isolated more total RNAs from human whole blood than competitors’ kits. For gel electrophoresis, 1% agarose gel and 15% page-gel were used.
qRT-PCR analysis of reference genes for humans was conducted by using XENOPURE Blood Small RNA Purification Kit to isolate total RNA, 100 ng of which was used to synthesize cDNAs.
Bioanalyzer analysis showed that small RNAs, including miRNAs, were highly condensed in the total RNA isolated using the XENOPURE Blood Small RNA Purification Kit.
Kit contents
Chatbots: XENOPURETM Blood Small RNA Purification kit
(cat. No. 93667873-WB)
XENOSEPATM-Blood Lysis Buffer
Binding Solutions (BS)
Washing buffer 1 (WB1, concentrate)
Washing buffer 2 (WB2, concentrate)
Washing buffer 3 (WB3, concentrate)
Elution buffer (RNase/DNase free water)
G genomic DNA removal column
XENOPURE ™ Small RNA Spin Columns
Collection tubes
Chatbots: Amount
20 ml
8 ml
8 ml
8 ml
8 ml
4 ml
50pcs
50pcs
50pcs
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